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p erk  (Bioss)


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    Bioss p erk
    P Erk, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+erk/PERK(Thr980)+Polyclonal+Antibody/pmc13096070-75-37-40
    Average 95 stars, based on 88 article reviews
    p erk - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: TRPC6 mediates neuronal hyperexcitability in the lateral habenula to drive trigeminal neuralgia-associated anxiety.
    Article Snippet: .. Primary antibody incubations TRPC6 (rabbit, 1:1000, 18236-1-AP, Proteintech, Wuhan, Hubei, China), βCaMKII (rabbit, 1:1000, NB120-22131, Novus Biologicals, Colorado, USA), CREB (rabbit, 1:1000, bsm-34317R, Bioss, Beijing, China), p-CREB (rabbit, 1:1000, bs-0036R, Bioss, Beijing, China), ERK (mouse, 1:500, bsm-332332M, Bioss, Beijing, China), p-ERK (rabbit, 1: 1000, bs-3016R, Bioss, Beijing, China), and GAPDH (1:20000, WJ103, YamayBio, Shanghai, China) were incubated at 4 ◦C overnight. .. After TBST washes (3 × 5 min), the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10000, EF0002, Sparkjade, Jinan, Shandong, China) or HRP-conjugated goat anti-mouse IgG (1:10000, EF0001, Sparkjade, Jinan, Shandong, China) for 1 h at room temperature (RT).

    Article Title: The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers
    Article Snippet: .. IF staining sections were incubated with p-AKT, (BIOSS, BS-0876R, 1:100) p-ERK (BIOSS, BS-2637R, 1:100), or anti-CD45 (Biolegend, 109837, 1:100) antibodies and were captured via Zessis. ..

    Article Title: The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers.
    Article Snippet: .. IF staining sections were incubated with p-AKT, (BIOSS, BS-0876R, 1:100) p-ERK (BIOSS, BS2637R, 1:100), or anti-CD45 (Biolegend, 109837, 1:100) antibodies and were captured via Zessis. ..

    Purification:

    Article Title: EML4-ALK G1202R and EML4-ALK L1196M mutations induce crizotinib resistance in non-small cell lung cancer cells through activating epithelial-mesenchymal transition mediated by MDM2/MEK/ERK signal axis.
    Article Snippet: Funding information The Innovative Talents Support Plan of Colleges and Universities in Liaoning Province, Grant/Award Number: LR2020080 Abstract Crizotinib, as the first‐generation of anaplastic lymphoma kinase (ALK) inhibitor, effectively improves the survival time of ALK‐positive non‐small cell lung cancer (NSCLC) patients.. However, its efficacy is severely limited by drug resistance caused by secondary mutations.. G1202R and L1196M are classical mutation sites located in ALK kinase domain.

    Staining:

    Article Title: The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers
    Article Snippet: .. IF staining sections were incubated with p-AKT, (BIOSS, BS-0876R, 1:100) p-ERK (BIOSS, BS-2637R, 1:100), or anti-CD45 (Biolegend, 109837, 1:100) antibodies and were captured via Zessis. ..

    Article Title: The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers.
    Article Snippet: .. IF staining sections were incubated with p-AKT, (BIOSS, BS-0876R, 1:100) p-ERK (BIOSS, BS2637R, 1:100), or anti-CD45 (Biolegend, 109837, 1:100) antibodies and were captured via Zessis. ..



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    Image Search Results


    GA effects on ERK1/2 phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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    doi: 10.3892/ol.2026.15541

    Figure Lengend Snippet: GA effects on ERK1/2 phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Mouse primary monoclonal antibodies directed against extracellular signal-regulated kinase (ERK)1 (clone G-8; cat. no. sc-271269), phosphorylated-ERK1/2 (p-ERK1/2; clone E-4; Tyr204 of ERK1; cat. no. sc-7383), OCT3/4 (clone A-9; cat. no. sc-365509) and RAGE (clone E-1; cat. no. sc-74473) were obtained from Santa Cruz Biotechnology, Inc.

    Techniques: Phospho-proteomics, Derivative Assay, Western Blot, Incubation, Expressing, Control